She went from selling hydration drinks out of the trunk of her car to building a $20M brand, without outside investors, industry experience or playing by the rules. 

In this episode, Jesslyn Rollins, the CEO of Biolyte, reveals how grassroots hustle, relentless sampling, and a medical-grade “IV in a bottle” helped her break into one of the most competitive categories in beverages, and why the brand is now ready to make its boldest move yet.

0:25: Interview: Jesslyn Rollins, CEO, Biolyte – At BevNET Live L.A. 2025, Jesslyn recounted Biolyte’s origins in 2016, when her father developed a medical-grade rehydration formula and she began selling it out of her car to high school athletic programs. She details how success with local football teams led to Biolyte’s big break into Kroger’s natural store sections, where cold placement and in-store sampling fueled rapid growth. Jesslyn talks about how Biolyte has expanded across regions, launched a rebrand, secured national powder-pack distribution in Walgreens and CVS, and positioned itself as a premium rapid rehydration sports drink with significantly higher electrolytes than legacy brands. She emphasizes the importance of consumer trust, data-driven storytelling, and evolving the brand’s message beyond niche use cases like athletics or illness to everyday wellness. Despite intense competition, operational challenges, and no outside investment, she stays motivated by customer testimonials and a clear mission, noting that Biolyte is now at an inflection point where incremental growth isn’t enough and bold strategic change is needed to become “the rehydration drink for the next generation.”

Biolyte, Gatorade, Powerade, 7UP, Poppi, BodyArmor